Coding

Part:BBa_K804010:Experience

Designed by: Joe Ho   Group: iGEM12_British_Columbia   (2012-10-02)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K804010

User Reviews

UNIQebb9d8f77bdb2c97-partinfo-00000000-QINU UNIQebb9d8f77bdb2c97-partinfo-00000001-QINU

MetA Knockout Complementation by British Columbia iGEM 2012

This part consists of an arabinose promoter, a strong RBS, and the MetA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added. 800px-MetA_complementation_no_fluor.png

To generate this graph we cultured an E. coli MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.